Zearalenone (ZON)

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Zearalenone (ZON)
  • Accreditation
    ISO 17025
  • Standard Turnaround Time
    10 working days

Fera Science Limited can analyse food or feed samples to measure levels of zearalenone using methods that are fully compliant with EU contaminants legislation. This allows you to assess compliance with the regulatory limits currently in force. Fera Science Limited also acts as UK National Reference Laboratory for mycotoxins in food and feed under Regulation (EC) No. 882/2004 advising food control laboratories in the UK on methodology and analysis.

The method used is fully accredited to ISO17025 for food and feed samples using an immunoaffinity column clean-up with HPLC-fluorescence detection. Extraction protocols have been optimised for many different matrices which, which coupled with highly specific clean-up protocols and detection produce results that are fit for purpose, accurate and precise. The method was fully validated by international method validation study by Fera and is the basis of the CEN standard method.

Fusarium fungi, is a class of commonly occurring fungi of northern temperate regions. Many Fusarium species can produce a number of different mycotoxins, one of which is zearalenone, which is commonly found on cereals grown in regions of America, Europe and Asia.

The major fungus responsible for producing zearalenone is Fusarium graminearum and grain infected with this organism will often be pink in color due to a pigment that can be produced by the fungus. However colour alone cannot be used to assess toxin levels in the crop.

Zearalenone is mainly found in maize but can also be found in wheat, barley, sorghum and rye. Fusarium species typically grow and invade crops in moist, cool conditions. In wheat, sorghum and corn, zearalenone occurs in preharvest grain, whereas in other commodities contamination can come from both pre- and post-harvest products. Variations in the incidence of zearalenone will occur with different crop years, cereal crop type and geographical areas.

Regulatory limits for zearalenone in food and feed are laid down in multiple EU regulations/recommendations and their amendments. These are: Regulation (EC) No 1881/2006 setting maximum levels for certain contaminants in foodstuffs; and Commission Recommendation 2006/576/EC on the presence of deoxynivalenol, zearalenone, ochratoxin A, T-2 and HT-2 toxins and fumonisins in products intended for animal feeding. The database product FC24 is an essential source of up to date legislative requirements (www.fc24.eu).

Submitting your sample

When submitting your sample please include the food sample submission form to allow us to process your samples efficiently. Failure to send a sample submission form may result in a delay of your sample being tested.

Download the food sample submission form here.


Serch for Specific Analytes



        Method Used

        Immunoaffinity column clean-up with HPLC-fluorescence detection.


        Yes - ISO 17025

        Sample Information

        50g minimum, maximum 20,000g

        Minimum Sample Quantity (Batch Size)


        Turnaround Time

        10 working days, 5 working day turnaround available upon request.


        10 µg/kg zearalenone

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