Diastase Test

SKU
FC2001
£125.00
Contact Us

  • Preferred contact Method:

  • Telephone

We will use the email address provided above to contact you about this service within 2 working days

Diastase Test
  • Method Used
    Spectrophotometry
  • Minimum Sample
    50g
  • Minimum no. of samples required
    5
  • Standard Turnaround
    10 working days

Can you be confident that your storage facilities aren’t degrading the quality of your products? Fera Science can perform analysis on honey samples for diastase enzyme activity (diastase number) giving you the confidence in the quality of your product using the official Phadebas® assay for determination of diastase activity.

To give you reassurance in the quality of your products, or your suppliers products should you consider carrying out a Diastase test.  Diastase enzymes are found within a range of foodstuffs including honey, which can convert starch to short-chain sugars, e.g. for use as energy. Enzyme activity and levels are directly linked to storage and conditions such as exposure to heat.  Heating honey degrades diastase enzyme but can be used to artificially increase short-chain sugar levels within this foodstuff resulting in artificial changes in taste, most notably sweetness. 

EU Council Directive 2001/110/EC of 20 December 2001 relating to honey lists diastase enzyme activity as one of the key composition criteria that have to be determined for honey intended for human consumption.

Determination of diastase activity is performed using a photometric method in which an insoluble blue dyed cross-linked type of starch is used as a substrate.  When this is hydrolysed by the diastase enzyme it produces blue, water-soluble fragments, which can be measured photometrically at 620 nm.  The absorbance of the solution is therefore directly proportional to diastase activity of the sample.

Results are expressed as Gothe units (or DN, diastase number) per g of honey.  The DN is defined as the amount of enzyme that will convert 0.01 g of starch to the prescribed end-point in 1 hour at 40°C under test conditions.

Submitting your sample

When submitting your sample please include the food sample submission form to allow us to process your samples efficiently. Failure to send a sample submission form may result in a delay of your sample being tested.

Download the food sample submission form here.

Detail

Specification

Method Used

Spectrophotometry

Accreditation

No

Minimum Sample Quantity (Batch Size)

5

Minimum Sample Weight

50g

Specific Sample Shipping Information

Honey sample must be in a plastic container with a screw lid. May require an import licence. The sample should not contain wax and just be a representative sample

Turnaround Time

10 working days

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