Bees and other pollinating insects perform essential services in any ecosystem. As well as providing a host of products such as honey, wax, propolis, royal jelly and venom, bees are critical pollinators. They pollinate around 70 of the 100 or so crop species that feed 90% of the world population, and honey bees are responsible for pollinating crops worth $30 billion a year. However, they are under serious threat. Various factors, including climate change, agricultural practices and parasites, such as the varroa mite, mean that bee populations across the world are in rapid decline. It’s now more important than ever to preserve these valuable insects. All participants in the food production supply chain need to work together to safeguard the health of bees and other pollinating insects.
How can Fera help?
Fera leads the way in pollinator safety research, testing and analysis. We offer a host of services to support the objectives of bee keepers, farmers, chemical producers, retailers and consumers. From standard laboratory studies to bespoke higher tier studies to address specific risk assessment needs, our experts at Fera are perfectly placed to meet any data requirements to help our partners develop products that are safe for bees and other pollinators.
Our role is to bring all participants in the food production supply chain together, sharing information and research, providing valuable testing services and creating opportunities for collaboration.
Our research expertise and scientific resources can help you to test your plant-protection products for their effects on bee survival, development and behaviour – helping you to develop products that are safe for bees and other pollinators. We work closely with the National Bee Unit, giving us access to a huge range of resources to support your risk assessments, including more than 150 colonies of honey bees and a highly skilled beekeeping team.
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At the forefront of bee ecotoxicology testing
Case Study - Radio Frequency IDentification tagging – Field Experiment
Open Field Studies
The tests detailed below demonstrate some of our capabilities. We will work with you to select the appropriate tests to meet the specific needs of your project. We can perform all of the necessary residue analysis for these studies, from testing dosing solutions to analysing complex hive matrices. If you have a specific testing requirement or problem that needs addressing, we can adapt our methods or design bespoke tests to provide the data, analysis and reports you need.
First tier tests designed to look at the worst case scenario via the two main routes of exposure: ingestion and direct contact. These tests are short term and are designed to generate LD50 and LOEC or NOEC values. Honey bee tests are according to the OECD Guidelines 213 & 214: Honey bees Acute Oral & Contact Toxicity Tests. We also test bumble bees according to OECD Test guidelines 246 and 246 Bumble Bee Acute Contact & Oral Toxicity. Unlike honey bees we are not restricted to testing during the beekeeping season – bumble bee laboratory tests can be performed earlier and later in the year.
Cage tests designed to assess possible longer-term effects of feeding on PPPs to honey bees. Test units of bees are dosed with a treated sucrose solution over a 10 day period; the feed uptake per day is calculated to determine the daily dose of the test item (according to OECD Test Guideline 245: Honey bee (Apismellifera L.), chronic oral toxicity test(10 day feeding test in the laboratory)2016. (Accepted in April 2016 at the OECD National Representatives meeting).
These tests assess the toxic effects of PPPs on honey bee larvae. The larvae are grafted from healthy colonies into artificial cells where they are fed an artificial diet(royal jelly and sugars) and reared in the laboratory. The test item is combined with the feed and fed directly to the larvae. These tests can be run as a single dose test or with repeated dosing (OECD Test Guideline 237: Honey bee larval toxicity test – Single exposure and OECD Guidance Document 239 Honey bee larval toxicity test – Repeated exposure).
This test is designed to assess the toxicity of residues of PPPs on plant material post application. Test item applications are made to a crop and are aged on the crop. The crop is collected at different time points and bees are exposed to the foliage. This test is performed according to EPA OCSPP850.3030: Honey Bee Toxicity of Residues on Foliage.
Fera has recently been developing protocols to investigate the honey bee metabolism of PPPs and toxicity of the metabolites to adult bees. These studies rely on our experience with bees backed up with high-end analytical chemistry capabilities on site.
These tests are run under tunnel conditions using a bee-attractive crop. A single colony is placed into each tunnel. Using photographic methods and bee brood monitoring software the development of different brood stages(eggs, young/old larvae) are monitored for any effects and the brood termination rate calculated (OECD 75: Honey bee brood test under semi-field conditions (tunnel test). Using photographic methods and bee brood monitoring software, it is possible to monitor the effects of PPPs on the development of different brood stages.
Tests performed according to OECD 75: Honey Bee Brood Test Under Semi-Field Conditions (tunnel test).
Similar to the bee brood test. However, the test item can be fed directly to free-flying honey bee colonies in sugar syrup. Additional measurements of mortality and overall colony development are also taken. Tests run according to Oomen, de Ruijter & Van der Steen (1992).
These are conducted under open field conditions, to give realistic exposure scenarios (EPPO 170) and can be designed to look at a number of aspects including: spray applications, seed treatments (e.g. dust) and risks from guttation. Residue analysis can be incorporated to look at residues within the crop and returning foragers (and also from within hive matrices). We are also experienced with the use of Radio Frequency Identification studies (RFID) in open field situations as recommended in the EFSA GD to assess potential effects of PPPs on the foraging behaviour and mortality of worker bees. This RFID technology can also be used for dose/release/homing flight tests and we are part of the current ring test group assessing these methods.
Although there are no recognised guidelines for bumblebee field studies, we have a great deal of experience of developing bespoke protocols so that we can work with bumble bees under field conditions.
Fera are members of the International Commission for Plant Pollinator Relationships (ICPPR) non-Apis working group helping to develop new test methods. We are also collaborating with Atlantic Pollination Ltd to extend our capabilities. Contact us directly about the latest developments.
As part of our role we provide support and advice to government running a wide variety of studies which investigate the effects of agro chemical (plant protection products) and new veterinary medicines (for beekeepers), nutrition and disease.
Chemical companies developing plant protection products for growers need to ensure their pesticides provide effective protection against pests and diseases, at the same time as safeguarding essential pollinators, such as bees.
There are over 250 species of bee in the UK, made up of 225 species of solitary bees, 25 bumble bees, and just one honey bee. Different species have different life cycles and are active at varying times throughout the year.
With a wide range of expertise in the diagnosis of honey bee pests and diseases and our work in preserving the future of our pollinators, Fera are the perfect partner for your diagnostic and research requirements.
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